Dr. Eldin Auis' seminar
Venerdì, 24 Maggio 2019 dalle ore 10:00 alle ore 11:30
Room 6302 - PRC building
SPEAKER: Dr. Hussam Hassan Nour Eldin Auis - DynaMo research center of excellence at Copenhagen Plant Science center - Copenhagen University Denmark
TITLE: Exploiting the potential of transporters in synthetic biology and Ag-Biotech
ABSTRACT: Transport Engineering” is an emerging research field aimed at improving biological production by controlling distribution of small compounds across membranes. Our work focus on developing tools to exploit the potential of transporters of plant specialized metabolites in synthetic biology and Ag-biotech. This area has remained unexplored due to lack of knowledge on transporters. I will present our breakthrough approach for identifying transporters of specialized metabolites and progress in two projects. In the first, we aim to remove the taste of mustard from mustard seeds. This project explores the concept of using Transport engineering to improve the nutritional value of crops by eliminating toxic defense compounds –specifically- from edible tissues without compromising plant fitness. We identified the first glucosinolate transporters in Arabidopsis via brute-force functional-genomics transporter-screening in Xenopus oocytes and demonstrated complete elimination of glucosinolates from seeds by mutating two transporters. We are in the process of translating this loss-of-function phenotype to Brassica juncea (mustard) where we have to overcome the challenge of polyploidy. In the second, we aim to boost production of phlorizin in yeast. In synthetic biology approaches, uptake of substrates, reimport of exuded intermediates and export of final products constitute inherent limitations on final yield. Although Arabidopsis does not produce phlorizin, we succeeded in identifying two transporters capable of exporting phlorizin by screening 553 Arabidopsis genes for transport activity. I will present the effect of introducing these transporters into a phlorizin producing yeast strain and how we have used the Arabidopsis transporters to identify the first bona fide phlorizin transporters from Apple.
HOST: Stefan Martens